How is eosin solution prepared

Eosin Y is a xanthene dye, an organobromine compound and a monocarboxylic acid used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains. Eosin is often employed in microscopy as a counterstain for visualizing proteins, connective tissues, fibers and keratin in sample material of human origin, and even for the routine staining of cryosections.

According to user requirements, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Stovall , W. Oxford Academic. Google Scholar. Cite Cite W. Select Format Select format. Permissions Icon Permissions. Article PDF first page preview.

You do not currently have access to this article. Download all slides. Mix well. Ammonium hydroxide concentrated 2 ml. Distilled water ml. Lithium Carbonate Solution Saturated :. Lithium carbonate 1. Eosin-Phloxine B Solution:. Eosin Stock Solution:. Eosin Y 1 g. Mix to dissolve. Phloxine Stock Solution:. Phloxine B 1 g. Eosin-Phloxine B Working Solution:. Eosin stock solution ml. Phloxine stock solution 10 ml. Glacial acetic acid 4 ml.

Alternate for Eosin-Phloxine B Solution. Eosin Y Solution:.